Pine bark extract reduces platelet aggregation

Mohsen Araghi-Niknam PImageDa, Saiid Hosseini MDa, Douglas Larson PImageDb, Peter Rohdewald PImageDc and Ronald Ross Watson PImageDCorresponding Author Contact Information, E-mail The Corresponding Author, a
a College of Public Health, University of Arizona, Tucson, AZ, USA
b Department of Surgery, University of Arizona, Tucson, AZ, USA
c Institute of Pharmaceutical Chemistry, Westfälische Wilhelms-Universität, Münster, Germany

Available online 14 September 2000.


The effects of long-term consumption of the bioflavonoid mixture, French maritime pine bark extract (Pycnogenol®), were assessed on aggregation of platelets from cigarette smokers and nonsmokers. Previously we showed that a single dose of Pycnogenol® reduced platelet aggregation in cigarette smokers in a dose–response fashion. Cigarette smoking increased platelet reactivity aggregation when measured 2 h after smoking the first cigarette of the day. Blood was collected immediately before and 5 min after smoking three cigarettes each. Smoking increased platelet aggregation (1.17 ± 0.04). However 200 mg Pycnogenol®/day, taken 3 h prior to first cigarette for the day for 2 months, significantly (p < .0023) reduced smoke-induced platelet aggregation (0.98 ± 0.05) to the level of nonsmokers. In a group of 19 nonsmokers, platelet aggregation was measured during in vitro stimulation by platelet aggregation factor (PAF) after 4 or 8 weeks of 200 mg/day of Pycnogenol® consumption. Platelet aggregation was significant when induced in vitro by PAF. However, Pycnogenol® consumption did not change platelet aggregation, suggesting that Pycnogenol®'s regulation of aggregation is by another mechanism. Thromboxane A2 (TxA2) is increased in smokers by release from platelets and rapidly becomes thromboxane B2 (TxB2). Smoking increased TxB2, which was prevented by Pycnogenol®, lowering TxB2 levels to those of nonsmokers. However, Pycnogenol® had no effect on the lower levels of TxB2 in nonsmokers. These observations suggest that Pycnogenol® supplementation reduces a risk factor for cardiovascular diseases, that is, platelet aggregation in smokers. The bioflavonoids in Pycnogenol® reduced platelet aggregation stimulated by tobacco smoke.

Author Keywords: platelet reactivity; bleeding; bioflavonoids

Corresponding Author Contact Information Address reprint requests to: Dr. Ronald R. Watson, College of Public Health, P.O. Box 245155, 1501 N. Campbell, University of Arizona School of Medicine, Arizona Health Science Center, Tucson, AZ 85724, USA; Fax: (520) 626-6093; email:

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